Figure 1 | Scientific Reports

Figure 1

From: Functional Neuronal Differentiation of Injury-Induced Muscle-Derived Stem Cell-Like Cells with Therapeutic Implications

Figure 1

In vitro differentiation protocol. Undifferentiated iMuSCs were cultured in Neural Stem Cell (NSC) Medium in suspension for 7 days to induce neurosphere formation. For myotube differentiation, neurospheres were plated on collagen-coated wells in Muscle Differentiation (MD) Medium. For neural differentiation, neurospheres were plated on laminin/poly-L-ornithine coated wells and cultured for 21 days in Neural Differentiation (ND3) Medium.

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