Figure 2 | Scientific Reports

Figure 2

From: Functional Neuronal Differentiation of Injury-Induced Muscle-Derived Stem Cell-Like Cells with Therapeutic Implications

Figure 2

Neurosphere formation of iMuSCs. (A) BF image of the control MuSCs that could not form neurospheres. (B) BF image of the iMuSC-formed neurospheres in suspension. (C–F) Immunofluorescence images of cryosectioned neurospheres after 7 days of culture. They stained positive for CNPase (red, C), Nestin (green, D), Nefm (red, E), and Gfap (green, F). Nuclei were stained with DAPI (blue). Scale bar = 100 µm. (F) Whisker plots summarize the gene expression profiles of five biological replicates of iMuSC-formed neurospheres after 7 days of culture compared to undifferentiated iMuSCs by qPCR.

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