Figure 1
From: Liver ERα regulates AgRP neuronal activity in the arcuate nucleus of female mice
AgRP mRNA synthesis does not fluctuate during the estrous cycle in LERKO mice. AgRP mRNA measured by Real Time PCR in the hypothalamus of floxed and LERKO mice during the different phases of the estrous cycle, protestrus (P), estrous (E), metestrus (M) and diestrus (D) (A), representative images of AgRP staining in the arcuate nucleus of floxed and LERKO mice at E and quantification of AgRP positive synaptic puncta as evaluated by IHC for AgRP protein in the ARC of floxed and LERKO female mice at E (B), NPY mRNA (C), CART mRNA (D), POMC mRNA (E) and KISS1 mRNA (F) measured by Real Time PCR in the hypothalamus of floxed and LERKO female mice in E and M. 3 V = third ventricle. The image of the brain slice in Fig. 1B shows the areas used for the morphological analyses (from −1.70 mm to −1.58 mm from bregma) and is adapted from the web site: http://www.hms.harvard.edu/research/brain/atlas.html. The morphometric analysis was done as described in the methodology section. Data shown are the combined result of 2 separate experiments each with the following number of animals: rtPCR: n = 4 mice/exp for floxed, n = 8 mice/exp for LERKO. IHC: n = 3 mice/exp for floxed, n = 5 mice/exp for LERKO, Data are represented as mean ± SEM. Figure 1A: **p < 0.01, two-way ANOVA followed by Bonferroni post hoc test, p = 0.0236, DF = 1, F = 5.84, LERKO vs floxed, °P < 0.05, p = 0.5918, DF = 3, F = 0.65, P vs E vs M vs D, but the interaction between the two variables is significant (p = 0.0284). Figure 1B: *p < 0.05, two-way ANOVA followed by Bonferroni post hoc test, p = 0.0342, DF = 1, F = 6, LERKO vs FLOXED, °p < 0.05, unpaired two-tailed t-test, p = 0.0147, DF = 1, t = 8.161, E vs M. Figure 1C: p = 0.3076, DF = 1, F = 1.09, LERKO vs floxed, p = 0.3555, DF = 1, F = 0.89, Fig. 1D: p = 0.5608, DF = 1, F = 0.36, LERKO vs floxed, p = 0.6709, DF = 1, F = 0.19, E vs M, Fig. 1E: p = 0.9114, DF = 1, F = 0.01, LERKO vs floxed, p = 0.3957, DF = 1, F = 0.79, E vs M, Fig. 1F: p = 0.4722, DF = 1, F = 0.54, LERKO vs floxed, p = 0.5109, DF = 1, F = 0.45, E vs M.
