Figure 3
From: Liver ERα regulates AgRP neuronal activity in the arcuate nucleus of female mice
Neither microglia morphology in the ARC nor AgRP synthesis is affected by HFD in LERKO mice. Iba1 positive cells (A) percentages of microglia distribution across different phenotypes (B) in the arcuate nucleus of floxed and LERKO female mice in M after 4 months of ND or HFD, representative images of microglia stained with an antibody against Iba1 in the arcuate nucleus of the hypothalamus of floxed and LERKO female mice in metestrus after 4 months of ND or HFD (C), AgRP mRNA measured by Real Time PCR in the hypothalamus (D) and AgRP positive synaptic puncta, as evaluated by IHC for AgRP protein, in the arcuate nucleus of the hypothalamus (E) of floxed and LERKO female mice in M, after 4 weeks of ND and HFD. Data shown is combined results of 2 separate experiments with the following n: rtPCR: N = 5 mice/group/exp. IHC: N = 5 mice/group/exp. Data are represented as mean ± SEM. Figure 3A: two-way ANOVA followed by Bonferroni post hoc test, DF = 1, F = 0.11, p = 0.7475, HFD vs ND, DF = 1, F = 0.41, p = 0.5329, LERKO vs Floxed. Figure 3B: °, #p < 0.05, *p < 0.01, two-way ANOVA followed by Bonferroni post hoc test, DF = 2, F = 12.75, p < 0.0001 A–C vs D–E vs F, DF = 3, F = 0, p = 1, LERKO vs SYN in each diet., Fig. 3D: °°p < 0.01, two-way ANOVA followed by Bonferroni post hoc test, DF = 1, F = 10.12, p = 0.0032 HFD vs ND, DF = 1, F = 0.89, p = 0.3512 LERKO vs Floxed. Figure 3E: °p < 0.05, two-way ANOVA followed by Bonferroni post hoc test, DF = 1, F = 10.14, p = 0.0058, HFD vs ND, DF = 1, f = 1.86, P = 0.1918, LERKO vs Floxed.
