Figure 4

Plasma of LERKO mice induced IL1β production in N9 microglia cells while TNFα, MCP1, MIP2 and IL10 mRNAs are unaffected. IL1β mRNA measured by Real Time PCR in N9 cells treated with 1% or 3% of mouse plasma of floxed and LERKO mice in E for 3 and 6 hours. (A). IL1β protein content in cell lysate from N9 cells treated with 3% of mouse plasma of floxed and LERKO mice in E for 6 hours (B), TNFα mRNA (C), IL10 mRNA (D), CD68 mRNA (E), MIP2 mRNA (F) measured by Real Time PCR in N9 cells treated with 3% of mouse plasma of floxed and LERKO mice in E for 6 hours. The dotted line represents the value of untreated N9 cells. Data shown are the combined results of 2 separate experiments (Fig. 4A,C,D and F: n = 4 wells/group/exp, Fig. 4B: n = 2 wells/group/exp), all data are represented as mean ± SEM. The dotted lines represent the values of untreated cells. Figure 4A: *P < 0.05, one-way ANOVA followed by Bonferroni post hoc test, DF = 1, F = 11.10, p = 0.0088, N9-LERKO vs N9-SYN - treatment with 1% plasma, DF = 1, F = 0.85, p = 0.3795 3 h vs 6 h – treatment with 3% plasma, ***p < 0.0001, DF = 23, t = 5.646, two-tailed unpaired t-test, N9-LERKO vs N9-SYN – 3% treatment. Figure 4B: **p = 0.0027, DF = 5, t = 5.516, two-tailed unpaired t-test. Figure 4C–F: no significant difference was found between the two experimental groups by two-tailed unpaired t-test, Fig. 4C: p = 0.3899, DF = 23, t = 0.8764, Fig. 4D: p = 0.2541, DF = 18, t = 1.178, Fig. 4E: p = 0.2541, DF = 9, t = 1.178, Fig. 4F: p = 0.9218, DF = 23, t = 0.09930.