Figure 5
From: Liver ERα regulates AgRP neuronal activity in the arcuate nucleus of female mice
TLR4 is involved in the IL1β production induced in N9 cells treated with plasma of LERKO mice. IL1β mRNA measured by Real Time PCR in N9 cells treated with 3% of mouse plasma of floxed and LERKO mice in E for 6 hours with or without prior treatment with TLR4 inhibitor TAK242 10 μM 30 min before the plasma. Data shown is combined results of 2 separate experiments (N = 4 wells/group/exp). The dotted line represents the value of untreated N9 cells. All data are represented as mean ± SEM. *p < 0.05, ***p < 0.001, one-way ANOVA followed by Newman Keuls post hoc test, p = 0.0007, DF = 4, F = 9.794.
