Figure 5
From: Tumor necrosis factor prevents Candida albicans biofilm formation
N,N-diacetylchitobiose blocks TNF effect on C. albicans biofilm. (a,b) N,N-diacetylchitobiose (Chi µg/mL) isolated or combined with hTNF or mTNF (ng/mL) or medium (−) was added to C. albicans ATCC 10.2.31 biofilm (metabolic activity − XTT assay); optical microscopy of biofilms grown with medium (c), medium + mTNF 20 (d), medium + mTNF (20) + Chi (100) (e), or medium + Chi (100) (f) for 48 h (Original x200); (g) filamentation index of biofilms after 48 h growth with medium, medium + hTNF (20), medium + hTNF + Chi (100) or medium + Chi (100); (i) metabolic activity after treatment with cellobiose (Cel), mannose (Man) or N-Acetylglucosamine (GlcNAc). Data represent the mean ± SEM Absorbance492nm of two independent experiments conducted in triplicate; *P < 0.001. Filamentation index represents the percentage of hyphae/blastoconidia in 10 hpf (Original x200).
