Figure 4
From: IVIG regulates the survival of human but not mouse neutrophils
Mouse polyclonal IgG does not induce cell death in mouse neutrophils. Flow cytometric characterization of C57BL/6 mouse neutrophil cell death of unprimed or primed cells by mouse polyclonal IgG (IgGMice). Representative example of Annexin V-FITC/PI staining assay (A) and statistical summaries (B) of Annexin−/PI−-, Annexin+/PI−- and Annexin+/PI+-gated cell populations are indicated for analysis after 15 h incubation with polyclonal mouse IgG. (C) Death of mouse C57BL/6 mouse (PMNBL/6) or BALB/c mouse (PMNBALB/c) neutrophils after IgG stimulation as assessed by ethidium bromide exclusion assay. (D) Binding profiles of IgGMice or IVIG to 609 glycan antigens, as assessed by glycan array technology. RFU = relative fluorescence units. Bars show mean ± SEM (n = 3). *p < 0.05. One way-ANOVA followed by Dunnets posttest. Specific death was calculated in comparison to untreated controls as outlined in the Materials and Methods section.
