Figure 5
From: The CsHSP17.2 molecular chaperone is essential for thermotolerance in Camellia sinensis
Purified recombinant CsHSP17.2 protein was separated on a 4–22% native gel (A) and the effect of CsHSP17.2 protein on the renaturation of chemically denatured citrate synthase (CS) (B). M, protein marker; lane 1: recombinant CsHSP17.2 protein. CS (15 μM) was denatured in 6 M guanidine hydrochloride for 2 h and then placed under refolding conditions containing 150 nM CsHSP17.2 proteins or H2O (Control). The remaining activity of CS is presented as the percentage of non-denatured CS activity. Error bars in figures represent standard errors generated from at least three replicate trials.
