Figure 1 | Scientific Reports

Figure 1

From: Oxidation of protein-bound methionine in Photofrin-photodynamic therapy-treated human tumor cells explored by methionine-containing peptide enrichment and quantitative proteomics approach

Figure 1

The scheme for Met-peptide enrichment. Reduced Met-peptides are labeled by the iodoacetyl-PEG2-biotin reagent under acidic conditions, whereas the oxidized Met-peptides are not. The labeled peptides are then captured and purified with streptavidin-Sepharose beads. After reduction by DTT, the captured Met-peptides are released from the beads and analyzed by LC-MS/MS.

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