Figure 1
From: PII Protein-Derived FRET Sensors for Quantification and Live-Cell Imaging of 2-Oxoglutarate

Schematic representation of the FRET-sensor constructs. Red: PII; Blue: mCerulean; Yellow: mVenus; White: linker regions. Flexible (FL) or stiff helical (HL) linkers or short linkers (SL) of different length, Chlamydomonas reinhardtii PII N-terminus (CrNT) or streptavidin affinity tag (Strep-tag). All Domains are presented to scale.