Figure 2
From: PII Protein-Derived FRET Sensors for Quantification and Live-Cell Imaging of 2-Oxoglutarate

Properties of FRET-sensor PII-TC3. (A) Relative NFRET response of PII-TC3 and PII-TC3-R9P to increasing 2-OG concentrations. (B) Influence of different ADP/ATP ratios on the NFRET response of PII-TC3 upon addition of 0.1 mM 2-OG. (C and D) Relative NFRET response of PII-TC3 (C) and PII-TC3-R9P (D), to different metabolites of the TCA cycle. Error bars: ±s.e.m., n = 4.