Figure 3
From: PII Protein-Derived FRET Sensors for Quantification and Live-Cell Imaging of 2-Oxoglutarate
fdGOGAT assay with complete and incomplete reaction mix. S. elongatus cells were grown to an OD750 of 0.3 in nitrate-supplemented medium. The 2-OG concentrations in the reaction mixture was determined by FRET over time. Results are shown for a complete reaction mix (black squares and black dashed line) and the blank mix lacking glutamine (white squares and red dashed line). Error bars: ±s.e.m., n = 3.
