Figure 3
From: High throughput single cell counting in droplet-based microfluidics
Counting of human cells. Bright field image (a) and fluorescence image (b) of HL60 cells encapsulated in droplets. Droplets were labeled by adding the soluble dye Sulforhodamine-B in the aqueous phase. Scale bar: 100 µm. (c) Distribution of HL60 cells in droplets (mean ± s.d for n = 3; Poisson fit is plotted as a straight line). Green triangles. Cell density was adjusted to 2 × 105 cells/mL such that expected theoretical cell to droplet ratio (λtheo) is λtheo = 0.1 (given that droplet’s volume is 500 pL). Cell distribution fitted λfit = 0.1 ± (7.4 × 10−4) with R2 = 0.99. Red circles. Cell density was adjusted to 106 cells/mL such that λtheo = 0.5. Cell distribution fitted λfit = 0.56 ± 0.01 with R2 = 0.99. Blue squares. Cell density was adjusted to 2 × 106 cells/mL such that λtheo = 1. Cell distribution fitted λfit = 0.96 ± 0.01 with R2 = 0.98. (d) Distribution of H1975 cells in droplets (mean ± s.d for n = 3; Poisson fit is plotted as a straight line). Green triangles. Cell density was adjusted to 2 × 105 cells/mL such that λtheo = 0.1. Cell distribution fitted λfit = 0.1 ± 0.006 with R2 = 0.99. Red circles. Cell density was adjusted to 106 cells/mL such that λtheo = 0.5. Cell distribution fitted λfit = 0.44 ± 0.006 with R2 = 0.99. Blue squares. Cell density was adjusted to 2 × 106 cells/mL such that λtheo = 1. Cell distribution fitted λfit = 1 ± 0.02 with R2 = 0.99.
