Figure 1
From: Identification of a nanobody specific to human pulmonary surfactant protein A
Human pulmonary tissues expressed abundant SP-A protein. (a) Representative image of Western blot for human pulmonary SP-A protein. Human pulmonary frozen tissues and human liver tissues were homogenized. Total proteins were extracted, separated (50 µg) on SDS-PAGE gel, and transferred to a PVDF membrane. The membrane was probed with the primary mouse anti-human SP-A monoclonal antibody (SP-A-mAb) and the secondary anti-mouse IgG-FITC antibody. (b) ELISA assay to detect human pulmonary SP-A protein. Human tissue protein extracts were added to 96-well plates, and the plates were incubated with SP-A-mAb and secondary antibodies. The absorbance at 450 nm (OD450) was determined in a microplate reader.
