Figure 2
From: Identification of a nanobody specific to human pulmonary surfactant protein A
Identification of nanobody that specifically bound to human lung SP-A protein. (a) A total of 15 positive monoclonal Nbs were identified. (b) Peptide sequencing analysis revealed 4 IgG2a subtype clones and 11 IgG3 subtype clones. (c) Cluster analysis of the 15 peptide sequences found 7 different sequences and 2 overlapping sequences (Nb4 and Nb28). Phage-ELISA assay was performed using human pulmonary tissue total protein extracts as the antigen source and SP-A-mAb as the primary antibody.
