Figure 4
From: Identification of a nanobody specific to human pulmonary surfactant protein A
Nb4 specifically reacted with human SP-A. (a) Representative image of Western blot showing that Nb4 specifically bound to human lung SP-A. Human pulmonary tissue total protein extracts were loaded on SDS-PAGE gel and transferred to PVDF membranes. The membranes were probed with SP-A-mAb, Nb4, and off-target Nb. (b) ELISA assay on human lung total protein extracts. Human lung tissue total protein extracts were added in 96-well plates. The plates were incubated with primary antibodies SP-A-mAb, Nb4, and off-target Nb and the corresponding secondary antibodies. (c) ELISA assay on recombinant human SP-A protein. Recombinant human SP-A protein was prepared and then was added to 96-well plates. The plates were then incubated with SP-A-mAb, Nb4, anti-Fc-His, and off-target Nb. ***P < 0.05, #P > 0.05.
