Figure 4 | Scientific Reports

Figure 4

From: Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Figure 4

Effect of lentinan on priming step of inflammasome activation and formation of Asc pyroptosome. (A) BMDMs were treated with lentinan (LNT) or LPS as the 1st signal, after which cells were replaced by media containing nigericin (NG, 2nd signal). Secretion of IL-1β was measured by ELISA, and Casp1 secretion and pro-IL1β expression were analyzed by immunoblotting. (B) LPS-primed BMDMs were transfected with dsDNA in the presence of LNT. Casp1 secretion and Asc pyroptosome formation were analyzed by immunoblotting using cell culture supernatants (Sup), cell lysates (Lys), and cross-linked pellets (Pellet) from whole cell lysates. (C) Recombinant human caspase-1 (rhCasp1) was incubated with its substrate (YVAD-pNA) in the presence of LNT as indicated. Bar graph presents the mean ± SD of relative fluorescence unit (RFU).

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