Figure 5 | Scientific Reports

Figure 5

From: Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Figure 5

Effect of lentinan on Listeria monocytogenes (LM)-induced peritonitis. (A) Mice (n = 3 for non-treated, n = 14 for LM- or LM+LNT−, and n = 6 for LNT-treated group) were ip-injected with Listeria monocytogenes (1,000 cfu in 200 μL of saline) with/without lentinan (LNT, 10 mg/kg). Number of peritoneal exudate cells (PECs) was calculated, and IL-1β, IL-6, and IL-18 secretion levels of peritoneal lavage fluids measured. (B) PECs were isolated from LM- and/or LNT-treated mice (n = 3 for group), and expression levels of TNFα, IL-6, IL-1α, Pro-IL-1β, and NLRP3 mRNAs were measured by RT-PCR. The bar graph (mean ± SD) indicates relative band density. (C) Peritoneal lavage fluids were collected from LM- and/or LNT-treated mice (n = 6 per group), and 10 μL of lavage was cultured onto BHI plates to assess peritoneal LM burden.

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