Figure 6 | Scientific Reports

Figure 6

From: Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production

Figure 6

Effect of lentinan on endotoxemic models. (A) Mice (n = 10 per group) were intraperitoneally (ip) injected with LPS (25 mg/kg) and/or lentinan (LNT) as indicated. Survival rates were observed at the indicated times. (B), LPS-primed BMDMs were transfected with LPS to activate non-canonical inflammasome in the presence of LNT as indicated. IL-1β and Casp1 secretion was analyzed by ELISA and immunoblotting. (B) LPS-primed BMDMs were infected with E. coli. (MOI = 10) to activate non-canonical inflammasome in the presence of LNT as indicated. Secretion of Casp1 was analyzed by immunoblotting, and the right bar graph indicates relative band density. Bar graphs present the mean ± SD. Lipo, Lipofectamine 2000TM.

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