Figure 3
Morphometric analyses of nuclei point towards nuclear pathology in soleus fibers from young DesR349P mice. (a) example images from the middle section of a single SOL fiber from a wt, het, and hom DesR349P knock-in mouse showing the SHG (grey) and counterstained nuclear signal (blue). Although the volume per nucleus in fibers from hom animals was not different to wt (b), other morphological parameters were significantly different, e.g. hom fibers showing an increased rounded shape (sphericity) (c), or vastly increased number of nuclei per fiber volume (d), the latter two also compared to het DesR349P mutations. The nuclear-to-myosin volume ratios were also largely increased in the hom DesR349P genotype (e). (c/n/m) depicts (c) cell nuclei in (n) fibers from (m) mice. *p < 0.05, **p < 0.01, one-way ANOVA with Bonferroni-correction. Scale bar: 20 µm.
