Figure 9
Lateral passive biomechanics properties from myoblasts derived from wt, het, and hom DesR349P mice. (a) Schematics of the magnetic tweezer experiments applying a constant magnetically-induced force of 10 nN for 3 s to myoblasts into which magnetic fibronectin-coated micro-beads had been coated to the membrane focal adhesion complexes. Shown are mean displacement curves during the ‘force on’ and ‘force off’ phase from which the stiffness and visco-elastic behavior were extracted. (b) Lateral compliance values (median values due to log-normal distribution) of the membrane and near-membrane cytoskeleton complex in 147 wt, 117 het, and 128 hom myoblasts as well as the visco-elastic parameter ß (mean values). (c) Percentage of detached beads during the ‘force-on’ phase.
