Figure 2
From: The Regulatory T-cell Transcription Factor Foxp3 Protects against Crescentic Glomerulonephritis
Foxp3 inhibits up-regulation of inflammatory cytokines and cell-mediated kidney injury in anti-GBM GN. Results of Real time PCR show renal inflammatory cytokines ((A) TNF-α and (B), IL-1β) and chemokine ((C), MCP-1) mRNA expression in normal and anti-GBM GN (day 14) in both WT and Tg mice. (D) Immunohistochemistry shows that macrophage (stained for F4/80+) infiltration within the kidney with anti-GBM crescentic GN on Day 14 after disease induction is markedly inhibited in Foxp3 Tg mice. (E) Immunohistochemistry shows that CD3+ T cell (stained with anti-CD3 antibody) infiltration within the kidney with anti-GBM crescentic GN on day 14 is notably inhibited in Foxp3 Tg mice. Each bar represents mean ± SEM. **p < 0.01 and ***p < 0.001 compared with normal mice; # p < 0.05 and ### p < 0.001 compared with the WT mice. n = 6–8 mice per group. Original magnification 200X (D,E).
