Figure 3

Overexpression of Foxp3 increases proportions of systemic and renal CD4⁺ Foxp3⁺ Tregs. (A) Results of two-color flow cytometry show that overexpression of Foxp3 increases proportions of splenic CD4⁺ Foxp3⁺ Tregs in normal Tg mice when compared with WT mice. Gated on CD4⁺ T cells. (B) Flow cytometry analysis of peripheral and splenic and renal infiltrated CD4⁺ Foxp3⁺ T cells from diseased WT and Tg mice.(C) Infiltration of CD4⁺ Foxp3⁺ Tregs in the inflamed kidney tissues on Day 14 after disease induction was identified by two-color immunofluorescence with Foxp3⁺ (green) and CD4⁺ (red). Examples of CD4⁺ Foxp3⁺ Tregs cells are indicated by white arrows. Each bar represents mean ± SEM. ^## p < 0.01 compared with the WT mice. n = 3 mice per group. Original magnification 200× (C).