Figure 5
From: Integrated single- and two-photon light sheet microscopy using accelerating beams
Maximum intensity projections of light sheet image stacks of a thin sagittal slice of fixed rat brain with Alexa Fluor 568 NCAM-immunolabelled axons imaged in two photon mode using the HighQ-2 laser. The brain slice was mounted on uneven surface. (a) Gaussian light sheet, (b) Airy light sheet (α = 7). (c) The same region imaged in single-photon Airy light sheet mode (excitation wavelength 488 nm, α = 7, deconvolved). Scale bars 20 μm, gamma adjusted for visualization. See Supplementary Videos 1–3.
