Table 1 Comparison of the experimentally determined axial resolution and FOV for each light sheet type in microns.

From: Integrated single- and two-photon light sheet microscopy using accelerating beams

Excitation Laser

Gaussian

Airy (α = 3.5)

Airy (α = 7)

Airy (α = 14)

Airy (α = 21)

Core (λ = 780 nm)

1.42 ± 0.03

1.86 ± 0.11

1.92 ± 0.03

2.22 ± 0.04

2.57 ± 0.11

HighQ-2 (λ = 1045 nm)

1.94 ± 0.02

2.11 ± 0.06

2.43 ± 0.09

2.64 ± 0.13

2.96 ± 0.19

FOV

47 ± 12

97 ± 17

153 ± 17

222 ± 42

288 ± 71

  1. The axial resolution is calculated as a mean FWHM of PSF as probed with 0.2 μm diameter green and red fluorescent microspheres. The FOV is averaged over results obtained with both the Core and HighQ-2 laser. The uncertainties are given at 95% confidence level.
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