Table 1 The proteins involved in the reactive oxygen species scavenging network of MB266.
Enzyme and reaction | Protein or domain | Accession | Description | 1-vs-2a | 3- vs-4 | 1- vs-3 | 2- vs-4 |
---|---|---|---|---|---|---|---|
Superoxide and dismutase (SOD) O2 − + O2 − + 2H+ → H2O2 + O2 | SodA | YP_491542.1 | Superoxide dismutase, Mn | 2.036 b | 1.756 | 1.144 | 0.967 |
SodB | YP_489920.1 | Superoxide dismutase, Fe | 0.677 | 0.999 | 0.85 | 1.202 | |
SodC | YP_489910.1 | Superoxide dismutase, Cu, Zn | — | — | — | — | |
Catalase 2H2O2 → 2H2O + O2 | KatE | YP_489993.1 | Hydroperoxidase HPII(III) | 0.874 | 1.155 | 1.19 | 1.599 |
KatG | YP_491509.1 | Catalase/hydroperoxidase HPI(I) | 1.002 | 1.062 | 0.943 | 1.007 | |
Glutathione reductase (GR) GSSG + NAD(P)H → 2GSH + NAD(P)− | Gor | YP_491935.1 | Glutathione oxidoreductase | 0.934 | 0.98 | 1.113 | 1.091 |
Glutathione peroxidase (GPX) H2O2 + 2GSH → 2H2O + GSSG | BtuE | YP_489972.1 | Glutathione peroxidase | 1.128 | 1.016 | 1.451 | 1.288 |
NADPH oxidase NADPH + e− + O2 → NADP− + O2 − + H+ ROS producer | AhpC | YP_488895.1 | Peroxiredoxin, alkyl hydroperoxide reductase | 1.049 | 1.087 | 0.99 | 1.087 |
AhpF | YP_488896.1 | Alkyl hydroperoxide reductase, large subunit | 1.099 | 1.152 | 1.064 | 1.083 | |
Thioredoxin and thioredoxin reductase H2O2 + Trxred → Trxox + 2 H2O | TrxA | YP_491658.1 | Thioredoxin 1 | 1.254 | 1.124 | 1.047 | 0.993 |
TrxB | YP_489160.1 | Thioredoxin reductase | 1.174 | 0.983 | 1.292 | 1.105 | |
YbbN | YP_488783.1 | Thioredoxin domain-containing protein | 1.067 | 0.889 | 1.405 | 1.163 | |
Ferredoxin | Fdx | YP_490753.1 | [2Fe-2S] ferredoxin | 1.337 | 0.672 | 1.249 | 0.621 |
Cytochrome H+ + Cytcred → Cytcox + OH− | CyoA | YP_488724.1 | Cytochrome c oxidase subunit 2 | 1.063 | 0.971 | 1.01 | 0.952 |
CyoB | YP_488723.1 | Cytochrome c oxidase subunit 1 | 1.03 | 1.122 | 1.055 | 1.01 | |
YhjA | YP_491917.1 | Cytochrome c peroxidase | 1.012 | 1.217 | 0.794 | 0.945 |