Figure 2 | Scientific Reports

Figure 2

From: Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection

Figure 2

Characterization of the recombinant C-KCE-HA/PrM-E. (A) Verification of HA and PrM-E insertion in C-KCE by PCR. The marker used was DL2000plus. Lane 1 is the DL2000plus marker. The products in lane 2, 3, 4, and 5 was amplified from samples with primer pairs JDPrM-E-F/JDPrM-E-R, and lane 6 and 7 are negative C-KCE and the H2O control. The products in lane 8, 9, 10, and 11 was amplified from samples with primer pairs JDHA-F/JDHA-R, and lane 12 and 13 are negative C-KCE and the H2O control. Full-length gel is presented in the Supplementary Figure 1. (B) Multiplestep growth curves of C-KCE-HA/PrM-E and C-KCE in CEF cells. (C) Detection of HA, PrM, and E proteins expressions in C-KCE-HA/PrM-E-infected CEF cells by western blot. The precursor PrM-E indicated by the red arrowhead. (D) Confirmation of the expression of HA and E protein in C-KCE-HA/PrM-E-infected CEF cells by immunofluorescence. CEF cells infected with C-KCE or mock-infected CEF cells were used as controls. Full-length blots are included in the Supplementary 2.

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