Figure 3 | Scientific Reports

Figure 3

From: Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection

Figure 3

Conferred protection from immunization of ducks with C-KCE-HA/PrM-E against virulent DEV challenge. Ducks were inoculated subcutaneously with 105 PFU of C-KCE-HA/PrM-E, 105 PFU of C-KCE, or PBS (n = 10 per group) as a control, then intramuscularly challenged with 100-fold duck lethal dose (DLD)50 HB/10 at 2 weeks (A), 4 weeks (B) post-vaccination (pv), respectively. Ducks were examined daily for 2 weeks after challenge.

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