Table 1 The gRNA coding sequences and targets sites.

From: Construction of a highly efficient CRISPR/Cas9-mediated duck enteritis virus-based vaccine against H5N1 avian influenza virus and duck Tembusu virus infection

gRNA ID

Targets sites

gRNA Coding Sequencea

gRNA-1

UL27 and UL26 gene junction

GGGTCCAATAACGACCGTCGTGG

gRNA-2

US7 and US8 gene junction

CTGGTTAACTGTATTATGCGCGG

gRNA-3

EGFP

GCTGAAGCACTGCACGCCGTAGG

gRNA-4

ERFP

CGGCCACGAGTTCGAGATCGAGG

  1. aUnderlined text shows the protospacer adjacent motif (PAM) sequence located on the immediate 3′ termination of the gRNA recognition sequence.
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