Table 2 Primers used for generating C-KCE-HA/PrM-E, identification of the C-KCE-HA/PrM-E, and determination the DTMUV load.
Primer ID | Sequence (5′ → 3′)a |
|---|---|
UL27-F | AGAAATATTACCCCTTCAAGTATATACT |
UL27-R | CCACACCCCACGAAAAATGGAAACACGGGGGA |
RFP-F | GTTTCCATTTTTATAATACAGTTAACCA |
RFP-R | CGGTTTCTTCGTGCGGCCGCTAGGGATA |
UL26-F | CCTAGCGGCCGCACGAAGAAACCGCCCAACG |
UL26-R | TCGGGGCCAGTGGTGCCTACTGGA |
US7-F | TACCGGATGTGTTTAAAACGACAGTTAT |
US7-R | TATTAATAACTAATAATACAGTTAACCA |
GFP-F | TAACTGTATTATTAGTTATTAATAGTAATCAA |
GFP-R | CAAACTCCGCGCCGCTTACAATTTACGCCTTA |
US8-F | AAATTGTAAGCGGCGCGGAGTTTGGAGT |
US8-R | AGGGCATGTGTTGTCAACTATAGGCTTC |
HA-1F | CTTCCCTCCCCCGTGTGGTGCTTT |
HA-1R | CACTATTTTCTCCATGGTGGCCTAAGGA |
HA-2F | TAGGCCACCATGGAGAAAATAGTGCTTC |
HA-2R | GCCCTGAGGCTACTTATCGTCGTCATCC |
HA-3F | GACGACGATAAGTAGCCTCAGGGCTAGA |
HA-3R | TGCGGTCGTGGCTATAGGAGGAAC |
PrM-E-1F | TATGATTGACTGTTTGCCTTTCAT |
PrM-E-1R | GTCGAGCATCTGCATGGTGGCGACCGGT |
PrM-E-2F | GTCGCCACCATGCAGATGCTCGACGGAC |
PrM-E-2R | GCGGCCGCTTTAGGCATTGACATTTACT |
PrM-E-3F | AATGTCAATGCCTAAAGCGGCCGCGACT |
PrM-E-3R | GACGAATCATTTATACGTGCGTGT |
JDHA-F | CCAAAGCTGTTGCGTCTC |
JDHA-R | AACACGTACAGTTTCGTT |
JDPrM-E-F | TTTGTACATGAGGTAATA |
JDPrM-E-R | CGTACCAATTGTTGAGGT |
NS1-F | GACACGGGGTGCTCAATCGACT |
NS1-R | AGCCATGACCTTTGATTTGATC |
QT-NS1-F | GGACAACAACAGCGAGTGGGAA |
QT-NS1-R | CATGTCGTCTCCGTGAGCAGTT |
DEV-F | AAATCTGCTTGCCGGGGATACC |
DEV-R | TGCGGCGAAGAGGCGCAAGCTA |
QT-DEV-F | TTTTCCTCCTCCTCGCTGAG |
QT-DEV-R | ACTTCTGCAAACCCGGCC |
