Figure 6 | Scientific Reports

Figure 6

From: Interplay between CCN1 and Wnt5a in endothelial cells and pericytes determines the angiogenic outcome in a model of ischemic retinopathy

Figure 6

Functional and regulatory relationship between CCN1 and Wnt5a in ECs. (A,B) ECs were transduced with Ad-CCN1 or Ad-luc adenoviral vector, allowed to adhere to cytodex beads and then embedded in fibrin gels in the absence and presence of Wnt5a. Sprout formation was monitored by microscopy after 3 days. Quantitative analyses of spheroid sprouting was determined and expressed in arbitrary units. (C,D) ECs were pretreated with 5-FU to inhibit cell proliferation and the effect on cell migration was determined with cell scratch test. A representative experiment of the percentage closure from cell front after 8 h is shown.

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