Figure 5 | Scientific Reports

Figure 5

From: Identification of Tp0751 (Pallilysin) as a Treponema pallidum Vascular Adhesin by Heterologous Expression in the Lyme disease Spirochete

Figure 5

Interaction of Tp0751-expressing B. burgdorferi with postcapillary venules in vivo. (a) Total bacterial interactions per minute with dermal PCVs of live mice, visualized by intravital microscopy (IVM). Representative IVM videos are presented in Supplementary Videos S4–S6. Numbers of bacteria that paused and moved more slowly over PCV surfaces under flow were manually counted in time-lapses acquired at 15 fps. (b) B. burgdorferi flaB DNA copy numbers in blood of mice collected after IVM, as measured by quantitative real-time PCR. (c) Copy number-adjusted interactions/min in PCVs. Numbers of mice (venules) analyzed per strain: parent: 14 (30); infectious: 8 (22); Bb-BBK32: 8 (24); Bb-Tp0751: 9 (25). Summary values represent means ± SEM for experiments performed with ≥4 independent bacterial cultures/strain. Statistics: one-way Kruskal-Wallis ANOVA with Dunn’s post-test. *Indicates p < 0.05 vs. parent.

Back to article page