Figure 4
From: ‘Gardos Channelopathy’: a variant of hereditary Stomatocytosis with complex molecular regulation
Current-voltage plots for the erythroid precursor cells of the healthy controls (n = 17, black circles) and II-4 patient (open or grey symbols). (A) Cells were exposed to a ramp protocol three times in a row. Each next hyperpolarization-depolarization cycle was followed by an increase in current at positive potentials in the EPCs of II.4 patient (n = 24, 19 and 12 for the ramps 1, 2 and 3), whereas for the cells derived from healthy donors it was never the case. (B) Increase in electric current at positive potential in cells of patient II.4 caused by repetitive ramp protocol application could be blocked by pre-treatment of cells with 15 µM methyl isobutyl amiloride (MIA, grey squares, n = 14), 15 µM TRAM-34 (black squares, n = 7) or omission of the extracellular Ca2+ (open triangles, n = 14). All I/V curves presented in the panel represent the values obtained for the third ramp protocol. Data are shown as mean ± SD.
