Figure 1
Changes of Tfh cell subsets in AMR. (a) CD4, CXCR5, IFN-γ, CXCR3 and CCR6 antibodies were used to detect Tfh1 cells; (b) CD4, CXCR5, IL-4, CXCR3 and CCR6 antibodies were used to detect Tfh2 cells; (c) CD4, CXCR5, IL-17, CXCR3 and CCR6 antibodies were used to detect Tfh17 cells; (d) CD4, CXCR5, ICOS, Foxp3 and CD127 antibodies were used to detect Tfh and Tfr cells; (e) CD4+CXCR5+ICOS+Tfh cell in lymphocyte (LYM) had no significant difference between AMR group and non-AMR group; (f) The ratio of IL-21-producing Tfh cells (CD4+CXCR5+IL-4+ CXCR3-CCR6-Tfh2 and CD4+CXCR5+IL-17+ CXCR3−CCR6+Tfh17 cell) in AMR group was significantly higher than that of non-AMR group; (f) The ratio of CD4+CXCR5+ICOS+FOXP3+CD127−Tfr cells in AMR group was significantly lower than that of non-AMR group. Two-sided Wilcoxon rank sum test: *p < 0.05 (n = 40) versus non-AMR. Values are mean ± SD.
