Table 1 Strains, plasmids and primers.
From: Policing the legume-Rhizobium symbiosis: a critical test of partner choice
Strain, plasmid, or primer | Genotype or sequence | Reference of source |
|---|---|---|
Strains | ||
Rlv3841 | Str derivative of R. leguminosarum bv. viciae strain 300 | |
RU3940 | Rlv3841 nifH::Ω Spr | This work |
3841 gusA | Rlv3841 with gusA marker | |
3841 celB | Rlv3841 with celB marker | |
OPS0365 | 3841 gusA nifH::Ω Spr | This work |
OPS0366 | 3841 celB nifH::Ω Spr | This work |
Plasmids | ||
pRU1907 | nifH cloned in pCR2.1 | This work |
pHP45Ω-Sp | Plasmid containing Ω spec cassette, Spr | |
pJQ200SK | pACYC derivative, P15A origin of replication, Gmr | |
pRU1908 | nifH::Ω, Spr in pJQ200SK | This work |
Primers | ||
pOTforward | CGGTTTACAAGCATAAAGC | |
p1002 | TTCCTCCATGTGCCTGGAGA | |
p1118 | GGTTCTTCGGAGTTTCTAT | |
oxp0460 | GCTTGATCATCGCCGGAAAC | |
oxp0461 | TGTCACCGCCGAAAACGATG |
