Figure 6
From: Engineered HSV vector achieves safe long-term transgene expression in the central nervous system
Transgene expression in different brain areas. Representative images (5 sections/animal, 4 animals/group) taken from coronal sections prepared from animals killed 2 weeks after JΔNI6 injection into the prefrontal cortex (A), striatum (B), Maynert nucleus (C) or substantia nigra (D). Native mCherry fluorescence in red; DAPI-stained nuclei in blue. Left panels are schematic drawings (adapted from Paxinos and Watson22 with permission from Elsevier) of the brain structures at the level of vector injection; anteroposterior coordinates relative to bregma are indicated. Central panels are images of sections corresponding to the area framed in green in the corresponding left panel. Right panels are higher magnifications of the areas framed in yellow in the corresponding central panel. Horizontal bars in A = 100 μm; horizontal bars in B–D = 500 μm.
