Figure 2
From: Molecular mechanisms underlying the evolution of the slp76 signalosome
Tissue expression patterns of bbslp76. (a) Relative expression levels of bbslp76 gene in various tissues of B. belcheri by qRT-PCR. 2-ΔΔCt values (normalized to endogenous control 18S) represented the expression of the bbslp76 gene in various tissues (muscle, intestine, gill, skin, ovum) of B. belcheri. Bars represent the average results of triplicate reactions for each tissue. Error bars represent the standard error of the mean (SEM) among three biological replicates. *, ** and *** indicate a significant expression level difference versus muscle as control at p < 0.05, p < 0.005 and p < 0.005, respectively. (b) bbslp76 polyclonal antibody titration (1:1000) in 293 T cells with exogenous expression of cMyc-bbslp76 and in amphioxus intestine lysate respectively. EV: Empty Vector. (c) and (d) Localization of bbslp76 in the gill (c) or intestine cells (d) of amphioxus. Amphioxus gill or intestine cells were bound to poly-L-Lysine-coated coverslips, fixed, and stained with anti-bbslp76 polyclonal antibody followed by a secondary fluorescent antibody (green). Nuclei were stained with DAPI (blue). Images were acquired using fluorescent microscopy at 400 times magnification. Bar = 2 μm.
