Figure 2 | Scientific Reports

Figure 2

From: Development of an inducible mouse model of iRFP713 to track recombinase activity and tumour development in vivo

Figure 2

Generation of an inducible iRFP knock-in mouse. (a) Scans from LI-COR PEARL imaging system of control, low or high dose tamoxifen induced R26CreERT2; LSL-iRFP mice. (b) Quantification of individual resected organs on a LI-COR Odyssey. R26CreERT2; LSL-iRFP mice were un-induced (control) or treated with a low or high dose of tamoxifen, sacrificed and the organs resected. First bar: control; second bar: low dose; third bar: high dose. Mean relative to control values (c) of 3 mice were plotted (bar) each point represents one specimen (3 per condition). (c,d,e) Primary mouse intestinal organoid cultures of R26CreERT2; LSL-iRFP animals were prepared and induced with 100 nM 4OHT. Images of representative crypts (c, identical LUTs) and per well quantification (d) as well as individual crypt quantification (e) are shown. (f,g) Pdx1-Cre; LSL-iRFP; KPC pancreatic tumour organoids were cultured and quantified daily. Example images are shown in (f) (identical LUTs) and quantification of 4 replicate wells is shown in (g).

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