Figure 3
iRFP is detectable by FACS analysis. (a) iRFP positive or control livers were strained, prepared in a doubling dilution, scanned and quantified. Cells were subsequently FACS analyzed in (b). (c) Quantification of iRFP positive cells from (b). (d) P48-Cre; LSL-iRFP; KPC pancreatic tumour sample or an iRFP negative control pancreas were digested, strained and prepared in a doubling dilution. (e) These cells were FACS analysed and plotted by iRFP intensity. (f) Quantification of iRFP positive cells from (e). Pdx1-Cre; LSL-iRFP; KPC pancreatic tumour organoids were cultured and scanned (g). (h) Single cells derived from organoids were FACS analysed and plotted by iRFP intensity. (i) Quantification of iRFP positive cells from (h).
