Figure 4
Analysis of tumour development in lung and pancreas by LSL-iRFP. (a) LSL- KRasG12D; Rosa26-LSL-MYC; LSL-iRFP mice were induced intranasally with Adeno-Cre and regularly imaged by PEARL. Images at day 20 post induction (upper) and endpoint (lower) are shown with identical LUTs. (b) The iRFP signal was quantified and plotted as baseline corrected values. (c) LI-COR Odyssey images of pancreas, gut and liver tissue of three Pdx1-Cre; LSL-iRFP mice. Identical LUTs were used for pancreas and stomach/duodenum. Liver tissue was detected at higher resolution. (d) Pdx1-Cre; LSL-iRFP (wt) and Pdx1-Cre; LSL-iRFP; KPC with Cre-inducible versions of 172H mutant p53 and oncogenic K-ras were imaged at the indicated time-points, 0 equals first day of imaging (average age approx. 8 weeks), and quantified until endpoint was reached for KPC animals. Wt animals were imaged again after approx. 200 days to exclude long-term changes in the iRFP signal. (e) Pdx1-Cre; LSL-iRFP; KPC mouse with detectable lung metastasis in the near-infrared image. Tumour and metastasis were resected and reimaged to confirm the source of the signal. The tissue was subsequently taken into culture, plated and quantified over time (f). Mean and SD (error bars) of 48 replicate wells are shown.
