Figure 5

Ripley’s K-statistics, analysing spatial clustering of microglia subtypes. (a) For all treatment groups, L(r) and H(r) derivatives of Ripley’s K-function are negative for small values of r, indicating that retinal microglia have a dispersed topographical organisation. (b) Upon ONC, defined as the minimum of H(r), the domain radius of microglia decreases in size (one-way ANOVA with Tukey’s post hoc test). (c) Comparison of cross-K (K _ij) and self-K functions (K _ii and K _jj) shows that ‘low activity’ and ‘high activity’ microglia subpopulations evenly decrease their domain radius after ONC injury (two-way ANOVA with Tukey’s post hoc test). Data are depicted as mean ± 95% confidence intervals.