Figure 5 | Scientific Reports

Figure 5

From: Paternal spatial training enhances offspring’s cognitive performance and synaptic plasticity in wild-type but not improve memory deficit in Alzheimer’s mice

Figure 5

Paternal spatial training for 1 week does not improve synapse- or memory- associated proteins in 3XTg F1 offspring. (a,b) Synapse-associated proteins in 7-m 3XTg F1 offspring measured by Western blotting. (c,d) Memory-associated proteins (CREB, p-CREB), human tau (HT-7), phosphorylated tau (PT231) and PP1 in hippocampus of 7-m 3XTg offspring measured by Western blotting. The blot images in (a–d) were cropped to display and quantitifed by the Odyssey Infrared Imaging System (Licor biosciences, Lincoln, NE, USA). Bar graphs show mean ± SEM. (two-tail t-test, n.s, n = 3 per group). (e) Immunohistochemical staining of SYT1 in cortex and hippocampus of 7-m 3XTg offspring brain slices. The HP panel: scale bar = 50 μm; the enlarged CA1, CA3 and DG: scale bar  = 2 μm. (f) SYT1 mRNA level in hippocampus of 7-m 3XTg offspring measured by quantitative RT-PCR. Bar graphs show mean ± SEM (two-tail t-test, n.s, n = 3 per group).

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