Table 1 Comparison of normalized fluorescence of cells carrying the different versions of putative 3-HP-inducible gene expression systems from P. putida KT2440 (Pp) and C. necator H16 (Cn) in the presence or absence of 10 mM 3-HP.

From: Characterisation of a 3-hydroxypropionic acid-inducible system from Pseudomonas putida for orthogonal gene expression control in Escherichia coli and Cupriavidus necator

Switchable system

E. coli MG1655

C. necator H16

Fluorescence intensity/OD600

Induction ratio

Fluorescence intensity/OD600

Induction ratio

Uninduced

Induced

Uninduced

Induced

PpMmsR/P mmsA

59 ± 3

721 ± 24

12.3*

444 ± 80

22,857 ± 1808

51.5*

PpHpdR/P hpdH

1,259 ± 68

29,351 ± 756

23.3*

304 ± 46

157,052 ± 8,409

516.6*

CnAraC/P acaD

0 ± 0

0 ± 0

0

311 ± 16

464 ± 69

1.5

CnHpdR/P mmsA1

22 ± 6

26 ± 5

1.2

847 ± 27

74,839 ± 1,763

88.4*

PpP mmsA

193 ± 16

243 ± 23

1.3

44 ± 8

42 ± 4

1.0

PpP hpdH

908 ± 60

1,077 ± 47

1.2

42 ± 1

40 ± 3

1.0

  1. Each system is composed of either a putative 3-HP-inducible promoter and its respective transcriptional regulator or a 3-HP-inducible promoter only. The mean values and standard deviations represent the normalized fluorescence of biological triplicates six hours after induction for E. coli MG1655 and C. necator H16. Asterisks indicate statistically significant induction values for p < 0.01 (unpaired t test).
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