Figure 6

APEX1 overexpression ameliorates phosphate-induced vascular calcification in vitro. Arithmetic means ± SEM of calcium content (a, n = 8; µg/mg protein), alkaline phosphatase activity (b, n = 8; units/mg protein) and of CYP11B2, PIT1, CBFA1 and ALPL relative mRNA expression (c, n = 8; a.u.) in HAoSMCs transfected with empty vector (V) or APEX1 (AX1) and treated with (Pi) or without (Ctr) phosphate and with or without additional treatment with 100 nM aldosterone (Aldo). *(p < 0.05), **(p < 0.01), ***(p < 0.001) statistically significant vs vector transfected HAoSMCs. ^†(p < 0.05) statistically significant vs Pi treated vector transfected HAoSMCs. ^§§(p < 0.01), ^§§§(p < 0.001) statistically significant between Pi and Pi + Aldo treated APEX1 transfected HAoSMCs.