Figure 7
Vascular osteoinductive transformation in adrenalectomized kl/kl mice is ameliorated by spironolactone. Arithmetic means ± SEM (n = 10–11; arbitrary units, a.u) of aortic Cyp11b2 (a) and Pit1, Cbfa1, Msx2 and Alpl (b) relative mRNA expression in wild-type (WT) and kl/kl mice following discontinuation of dietary rescue and treatment without or with spironolactone (Spr) and adrenalectomy (Adx) for 5 weeks. *(p < 0.05), **(p < 0.01), ***(p < 0.001) statistically significant vs. WT control mice. †(p < 0.05), ††(p < 0.01) statistically significant between kl/kl-Adx and kl/kl-Adx + Spr. (c) Representative confocal microscopy images showing Cyp11b2, Cbfa1 and Msx2 protein expression in aortic tissues from wild-type (WT) and kl/kl mice following discontinuation of dietary rescue and treatment without or with spironolactone (Spr) and adrenalectomy (Adx) for 5 weeks. Images are representative of four mice per group. Protein expression: green labeling; nuclei: blue labeling and actin staining: red labeling. Scale bar: 20 µm.
