Figure 4

Schematic of the structures of measurement system and the measurement of local cellular temperature. (a) In normal practice, the testing device is put into the incubator at 37 °C and the other measurement instruments are put on the outside of the incubator at 25 °C. (b) The testing device and the other measurement instruments are put into the incubator at 37 °C, leaving the computer and monitor outside of the incubator at 25 °C. (c) A big constant-temperature tent kept at 32 °C is put on the outside of the incubator. Testing device and measurement instruments are put inside the incubator. The CO2 gas tank, computer and monitor are put on the outside of the tent. (d) A live cell firmly sticks to the micro-TFTC sensor. (e) A non-uniform temperature distribution in the cell (15–25 μm in diameter).