Table 1 The primers used in this study.
Primer | Sequence (5′ to 3′) | Usage | Reference |
|---|---|---|---|
TaPIMP2-fl_F | TGCCTAGCTCGTGGGAGTAG | Full-length cloning of TaPIMP2 | This study |
TaPIMP2-fl_R | CAATGGTTCTTTGCTGTCCTG | ||
TaPIMP2-GFP_F | ATGTCGACATGGGACGTCCGTCGTC | Construction of subcellular localization vector | This study |
TaPIMP2-GFP_R | GTCGGATCCGAAGTATGGTTCCAATTCC | ||
TaPIMP2-NheIF | CATGCTAGCCTCCGAGAATCTGGGCTACG | Construction of VIGS vector | This study |
TaPIMP2-NheIR | CTGCTAGCCCGGGGAGAAAGAAAGAAGA | ||
TaPIMP2-OE_F | ATACTAGTATGGGACGTCCGTCGTC | Construction of transformation vector | This study |
TaPIMP2-OE_R | GTCGAGCTCGAAGTATGGTTCCAATTCC | ||
TaPIMP2-TRANS_F | TTTTGATTTCAACTTGGAATTGG | PCR detection and ddPCR of introduced TaPIMP2 | This study |
TNOS-R | ATGTATAATTGCGGGACTCTAAT | ||
TaSTOP1A-F | GCAGAGGAGCGAGGCGATGGACGAC | ddPCR of reference TaSTOP1-A | This study |
TaSTOP1A-R | GCTGCAAGAACCCGGTCC TGAAG | ||
TaPIMP2-1A_qF | AACAGAGCCTCCTCGCAAGT | Quantification of TaPIMP2-1A | This study |
TaPIMP2-1A_qR | AAGAAGGTAAAAAATGGAGGGAA | ||
TaPIMP2-1B_qF | AACAGAGCCTCCTCGCAAGT | Quantification of TaPIMP2-1B | This study |
TaPIMP2-1B_qR | GGTGGGAATCGAGATAATTGG | ||
TaPIMP2-1D_qF | CCGCTAGAGCACAAGTTGTCG | Quantification of TaPIMP2-1D | This study |
TaPIMP2-1D_qR | GGGTGGGAATCGAGATAATTCA | ||
TaPIMP2-qF | GCATTGTACGGCCAGTTCG | Quantification of TaPIMP2 | This study |
TaPIMP2-qR | CGAGGAGGCTCTGTTCTTGG | ||
TaACTIN-qF | CACTGGAATGGTCAAGGCTG | Quantification of TaACTIN | Zhang et al.18 |
TaACTIN-qR | CTCCATGTCATCCCAGTTG | ||
PR1a-qF | CGTCTTCATCACCTGCAACTA | Quantification of PR1a | Zhang et al.18 |
PR1a-qR | CAAACATAAACACACGCACGTA | ||
PR2-qF | CCGCACAAGACACCTCAAGATA | Quantification of PR2 | Zhang et al.18 |
PR2-qR | CGATGCCCTTGGTTTGGTAGA | ||
PR5-qF | ACAGCTACGCCAAGGACGAC | Quantification of PR5 | Ameya et al.39 |
PR5-qR | CGCGTCCTAATCTAAGGGCAG | ||
PR10-qF | CGTGGAGGTAAACGATGAG | Quantification of PR10 | Zhu et al.25 |
PR10-qR | GCTAAGTGTCCGGGGTAAT |
