Table 1 Alignment of the deduced amino acid sequences of goldfish steroidogenesis enzyme genes with those of other animal species.

P450scc

JQ340311

91% (Gobiocypris rarus)

84% (Tautogolabrus adspersus)

81% (Oryzias latipes)

75% (Danio rerio)

3-beta-HSD

JQ867353

91% (Gobiocypris rarus)

Partial sequence of cofactor binding motif: Gly–X–X–Gly–X–X–Gly¹¹; Enzyme active site of short-chain alcohol dehydrogenase: Tyr–X–X–X–Lys

87% (Danio rerio)

77% (Oncorhynchus mykiss)

76% (Clarias gariepinus)

20-beta-HSD

KC193327

97% (Cyprinus carpio)

Adenine ring binding domain: X-Asp-X-X-Asp; Sequence for a β sheet: Gly-Gly-X-Asp-X-X-X-Asn-Asn-Ala-Gly-X; Active site: Thr-Asn-X-X-Gly-Thr-X-X-X-X-X; Substrate binding site: X-X-Asn-X-Ser-Ser-X; Enzyme active site: Tyr-X-X-X-Lys^{12, 13}; Sequence regulating hydrogen bonds in nicotinamide ring: X-X-X-Asn-X-X-X-Pro-Gly-X-X-X-Thr¹⁴

90% (Danio rerio)

80% (Tachysurus fulvidraco)

79% (Oncorhynchus mykiss)

17-beta-HSD1

JX036034

86% (Danio rerio)

Coenzyme binding domain: Thr-Gly-X-X-X-Gly-X-Gly; Connection between coenzyme binding domain and substrate activation site: Asn-Ala-Gly; Enzyme active site: Tyr–X–X–Ser–Lys with Ser and Asn upstream; Sequence III directing reaction

71% (Anguilla japonica)

70% (Oreochromis niloticus)

P45011beta

KC193328

85% (Danio rerio)

Steroid binding region; Oxygen binding region; Ozols’ region

81% (Oreochromis niloticus)

80% (Oryzias latipes)

74% (Oncorhynchus mykiss)

11-beta-HSD2

KC193326

91% (Danio rerio) 82% (Anguilla japonica) 81% (Oncorhynchus mykiss) 80% (Clarias gariepinus) 78% (Oryzias latipes)

Coenzyme I binding domain: Gly-Phe-Gly; Conserved sequence of 11-beta-HSDs: Cys-Met-Glu-Val-Asn-Phe-Phe-Gly; Enzyme active site: Tyr-Gly-X-Ser-Lys-Ala-Ala