Table 2 Primers for PCR amplification.
Gene | Primer | Primer sequence (5′~3′) | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
P450scc | Sense | GTNYTGTATGGGGARCGTYTG | 50.6 | ≈500 |
Anti-sense | CWGGATGTAAYCTBAGWGTTTC | |||
3-beta-HSD | Sense | TGTGTGTGGTGACAGGAGCC | 60.0 | 824 |
Anti-sense | CCCAAAGCCTAATGGTGAAAG | |||
20-beta-HSD | Sense | TGGCGATTGTGAAAGGGTTG | 56.6 | 690 |
Anti-sense | CTTCCTCTGGACTCTTGGTAGCAT | |||
17-beta-HSD1 | Sense | GGATTGGACTCAGCCTTGC | 58.4 | 698 |
Anti-sense | CATCGCCTCCAGATACACCT | |||
P45011beta | Sense | GAGCTGTCACGTTCTGTACGGC | 63.5 | 532 |
Anti-sense | TGTCTCCTTGATGGTCCCCTTC | |||
11-beta-HSD2 | Sense | TTCAGGTTTTGGTAATGCTACA | 53.0 | 697 |
Anti-sense | GTCGATGACAGGACTCAGGTC |
